Fig 1: Immunofluorescence and WB analyses of apoptosis-related proteins in mouse hippocampal tissue. (A) Representative immunofluorescence images and (B) densitometric analysis of active caspase-3; both 10 and 25 nM/kg semaglutide decreased the fluorescence intensity of active caspase-3 in the CA1 and CA3 regions (one-way ANOVA). (C) Representative immunofluorescence images and (D) densitometric analysis of Bax showing that both doses of semaglutide decreased the fluorescence intensity of Bax in the CA1 and CA3 regions (one-way ANOVA). (E) Representative immunofluorescence images and (F) densitometric analysis of Bcl-2 showing that both 10 and 25 nM/kg semaglutide increased the fluorescence intensity of Bcl-2 in the CA1 and CA3 regions (one-way ANOVA). (G) Representative WB images of active caspase-3, Bax and Bcl-2 in the different groups. (H and I) Statistical results of the immunoblot analysis showing that 10 and 25 nM/kg semaglutide reduced the band intensity of active caspase-3 (one-way ANOVA) and increased the Bcl-2/Bax ratio (two-way ANOVA). Data are presented as the means ± SD. *P<0.05, **P<0.01 and ***P<0.001. N≥3. WB, western blot/blotting; PTZ, pentylenetetrazole.